Morning loaded calorie intake in humans has been advocated as a dietary strategy to improve weight loss. This is also supported by animal studies suggesting time of eating can prevent weight gain. However, the underlying mechanisms through which timing of eating could promote weight loss in humans are unclear. In a randomized crossover trial, 30 subjects with obesity/overweight underwent 2 x 4 week calorie restricted, but isoenergetic weight loss diets, with morning loaded, or evening loaded calories (45:35:20% versus 20:35:45% calories at breakfast, lunch and dinner, respectively). We demonstrate no differences in total daily energy expenditure or RMR related to the timing of calorie distribution, and no difference in weight loss. Participants consuming the morning loaded diet reported significantly lower hunger. Thus, morning loaded intake (big breakfast) assists with compliance to weight loss regime, due to a greater suppression of appetite.