Document details for 'A high intake of industrial or ruminant trans fatty acids does not affect the plasma proteome in healthy men'

Authors de Roos, B., Wanders, A.J., Wood, S., Horgan, G.W., Rucklidge, G., Reid, M., Siebelink, E. and Brouwer, I.A.
Publication details Proteomics 11(19), 3928-3934.
Abstract Consumption of industrial trans fatty acids raises the risk of cardiovascular disease, but it is unclear whether cis9,trans11 conjugated linoleic acid (CLA) - a trans fatty acid derived from biohydrogenation in ruminant animals - modulates disease development. We investigated effects of industrial trans fatty acids or cis9, trans11 CLA, compared with oleic acid, on regulation of plasma proteins in healthy men. Twelve men were sequentially fed each of three diets for three weeks, in random order, for a total of nine weeks. Diets were identical except for 7% of energy which was provided either by industrial trans fatty acids, cis9, trans11 CLA or oleic acid. Plasma was collected at the end of each 3-week intervention period, depleted of its 12 most abundant proteins and analyzed by 2-D gel electrophoresis. Principal component analysis of protein spot intensity values revealed that the nature of the dietary intervention did not significantly affect the plasma proteome. The intervention provided in the 1st period produced a significant treatment effect compared with the interventions being provided in the 2nd and 3rd period, and there was also a significant subject effect. These findings were confirmed by the distribution of p-values of tests of treatment, period and subject effects. In conclusion, the nature of an extreme dietary intervention, i.e. 7% of energy provided by industrial trans fat or cis9,trans11 CLA, did not markedly affected the plasma proteome, thus plasma proteomics appears, by and large, an unsuitable approach to detect regulation of plasma proteins due to changes in the diet.
Last updated 2013-09-19

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