ANALYSIS AND OUTPUT FILE FROM AMOVA

The P-value of 0.002 indicates that the value obtained for the variance is the largest of the 500 permutations.

So we can conclude that the variance among populations is greater than 0.

Stated another way, the correlation between two clones randomly chosen from a subpopulation is significantly greater than the correlation between two clones randomly chosen from the whole group.

                       ANALYSIS OF MOLECULAR VARIANCE
                       ==============================
Input files
===========

 Distance Matrix File          : C:\AMOVA\POPLARS\RPOPS.DIS
 Group File                    : C:\AMOVA\POPLARS\POPLAR.GRP
 Population File generic name  : poppop

 No. of Groups      :     1
 No. of Populations :     6
 No. of Chromosomes :    35
 No. of Haplotypes  :    35

Sum of squares
==============

                SS(A) =      94.0444
                SS(B) =      88.7556
                ====================
            SS(Total) =     182.8000

Within Population sums of squares
=================================
 (Population    1)      20.8889
 (Population    2)      13.2000
 (Population    3)       3.0000
 (Population    4)      20.8750
 (Population    5)       4.6667
 (Population    6)      26.1250


Within Group sums of squares
=============================

 (Group    1)     182.8000


Mean squares
============

 d.f.(A) =     5    MS(A) =   18.809
 d.f.(B) =    29    MS(B) =    3.061
           =====
 Total        34

Variance components
===================

 Variance among populations    V(A) :  2.81795673400  (47.94%)
 Variance within populations   V(B) :  3.06053639850  (52.06%)

 PHI-statistics :   PHIst =  0.479


Distances among populations
===========================

 Distances = PhiST between pairs of populations

 Above diagonal    : Probability  Random distance (PhiST) > 
                                           Observed distance 
 Nb. of iterations :    500

       1       2       3       4       5       6
  0.0000  0.0000  0.0000  0.0000  0.0000  0.0000
  0.6034  0.0000  0.0000  0.0000  0.0898  0.0000
  0.5726  0.5329  0.0000  0.0000  0.0000  0.0000
  0.2861  0.6214  0.5024  0.0000  0.0000  0.0000
  0.4766  0.6735  0.6722  0.4418  0.0000  0.0000
  0.4220  0.3867  0.3522  0.3790  0.5306  0.0000


 Distances = Modified Coancestry coefficient
 (-ln[1-PhiST]=t/2N) between pairs of populations  

 Above diagonal    : Probability  Random distance (PhiST) > 
                                             Observed distance 
 Nb. of iterations :    500

       1       2       3       4       5       6
  0.0000  0.0000  0.0000  0.0000  0.0000  0.0000
  0.9248  0.0000  0.0000  0.0000  0.0898  0.0000
  0.8501  0.7612  0.0000  0.0000  0.0000  0.0000
  0.3370  0.9713  0.6979  0.0000  0.0000  0.0000
  0.6474  1.1192  1.1154  0.5831  0.0000  0.0000
  0.5481  0.4888  0.4342  0.4765  0.7564  0.0000



Variance Components Significance
================================

No. of permutations : 500

                      P(More extreme        No. of different
                       random value)    values in distribution
  VarA  and PhiST :      <0.0020                   84



Variance components null distributions
======================================

Var(A) and PhiST null distributtions saved in 
C:\AMOVA\POPLARS\SMARTVA.NUL

AMOVA Version 18.6.96 Page : 10e of 12