Biomathematics & Statistics Scotland
We have developed software to estimate gene expression by combining multiple scans of the same microarray
at different laser intensities. By combining the scans, we can reduce certain undesirable effects: firstly, we can
detect weakly expressed genes whose signal is lost in noise when the laser intensity is too low; secondly, we
can detect strongly expressed genes whose signal is
censored when the laser intensity is too high. This
software can also be used combine multiple replicates
at identical laser intensities, helping to reduce the
impact of measurement inconsistencies.
