Food nutritional labeling is compulsory in the European Union since 13 December 2016. The olive oil fatty acid composition shows high variation depending mainly on the variety. Thus, olive oil nutritional labeling is problematic for the industry. Besides, the analysis of all batches of olive oil using the official methods is expensive.Therefore, the olive oil industry is seriously concerned about solutions for nutritional labeling. In this study, a new rapid technique to measure the nutrients for the olive oil nutritional labeling, is assessed. A novel partial least squares (PLS) calibration model using log-ratio coordinates has been formulated and successfully tested for predicting the percentages of monounsaturated, saturated, and polyunsaturated fatty acids based on visible and near infrared spectroscopy. The model provided accuracy suitable for labeling, under the rules in force in the European Union. The error was generally much lower than the tolerance. Industrial relevance:The approach here proposed can be a suitable solution for olive oil nutritional labeling, which is a current challenge for the olive oil industry.