Moving from conventional towards sustainable agricultural cropping systems is important to reduce the environmental footprint of crop production by minimising the use of fossil fuel derived inputs and substituting these where required by renewable resources, without compromising crop yield and their nutritional value. In 2009, the Balruddery Farm Platform was established at The James Hutton Institute as a long-term experimental platform for cross-disciplinary research on sustainability in agricultural ecosystems. Crops representative of UK (and Scottish) agriculture were grown under conventional and sustainable conditions and analysed for their water soluble vitamin content as an indicator for their nutritional quality. Sustainable over conventional treatments only had minor effects on vitamin content, but significant effects were seen for thiamine in field beans (p<0.01), spring barley (p<0.05) and winter wheat (p<0.05), and for nicotinic acid in spring barley (p<0.05). However, for all crops, cultivar and inter-year differences were of greater importance. These results indicate that sustainable cropping systems do not compromise nutritional value in terms of water soluble vitamin content.