Document details for 'Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry'

Authors McLean, K., Palarea Albaladejo, J., Currie, C.G., Imrie, L.H.J., Manson, E.D.T., Fraser-Pitt, D., Wright, F., Alexander, C.J., Pollock, K.G.J., Allison, L.J., Hanson, M.F. and Smith, D.G.E.
Publication details Talanta 182(2018), 164-170. Elsevier.
Publisher details Elsevier
Keywords STEC, MALDI, Mass spectrometry, Escherichia coli, Enterohemorrhagic E. coli, EHEC, Shigatoxigenic E. coli
Abstract Whole cell MALDI is regularly used for the identification of bacteria to species level in clinical Microbiology laboratories. However, there remains a need to rapidly characterize and differentiate isolates below the species level to support outbreak management. We describe the implementation of a modified preparative approach for MALDI-MS combined with a custom analytical computational pipeline as a rapid procedure for subtyping Shigatoxigenic E. coli (STEC) and accurately identifying strain-specifying biomarkers. The technique was able to differentiate E. coli O157:H7 from other STEC. Within O157 serotype O157:H7 isolates were readily distinguishable from Sorbitol Fermenting O157 isolates. Overall, nine homogeneous groups of isolates were distinguished, each exhibiting distinct profiles of defining mass spectra features. This offers a robust analytical tool useable in reference/diagnostic public health scenarios to support management of outbreaks.
Last updated 2018-05-07

Unless explicitly stated otherwise, all material is copyright © Biomathematics and Statistics Scotland.

Biomathematics and Statistics Scotland (BioSS) is formally part of The James Hutton Institute (JHI), a registered Scottish charity No. SC041796 and a company limited by guarantee No. SC374831. Registered Office: JHI, Invergowrie, Dundee, DD2 5DA, Scotland