Cane splitting, a normal feature of raspberry growth, can lead to plant infestation by cane midge followed by fungal infection, with losses in yield of up to 50% if left untreated. The extent of splitting in the 'Latham'  'Glen Moy' mapping population was assessed over six years and in three environments and quantitative trait loci (QTL) were identified across five linkage groups (LG). Cane splitting QTL on LG 2, 3 and 5 co-locate with QTL for plant vigour but the cane splitting QTL on LG 4 and 6 are not associated with the plant vigour QTL on these LGs. Three genes, a carbon catabolite repressor-4 associated factor (CAF1), allene oxide cyclase and constitutive triple response 1 (CTR1), which are involved in general plant growth and in defence responses lie within these QTL. This is a first step towards identifying the genetic basis of cane splitting in raspberry and the development of genetic markers for use in raspberry breeding programmes.