Document details for 'Effect of supplementation with an 80:20 cis9, trans11 conjugated linoleic acid blend on the human platelet proteome'

Authors Bachmair, E-M., Bots, M.L., Mennen, L.I., Kelder, T., Evelo, C., Horgan, G.W., Ford, I. and de Roos, B.
Publication details Molecular Nutrition & Food Research 56(7), 1148-1159.
Keywords Conjugated linoleic acid Platelet proteomics Human nutrition intervention
Abstract Conjugated linoleic acids (CLA) improve the functioning of vascular cells including endothelial cells, monocytes and platelets. These cells all play an important role in the pathogenesis of atherosclerosis. In this study we assessed how supplementation of the diet with cis9,trans11-CLA affect mechanisms of human platelet function in vivo using a platelet proteomics approach. In a double-blind, randomized, placebo controlled parallel-group trial 40 overweight but healthy adults received either 4 g per day of CLA-enriched oil or placebo oil for three months. Proteins were extracted from washed platelets obtained just before and at the end of the intervention period. Cytosolic platelet proteins were separated using 2-dimensional gel electrophoresis and analysed for differences in spot abundance. Differentially regulated protein spots were identified by LC-MS/MS. Supplementation with cis9,trans11-CLA compared with placebo resulted in significant alterations in levels of 46 proteins (p<0.05), of which 35 were identified. Pathway analysis revealed that the majority was involved in regulation of the cytoskeleton and platelet structure, as well as receptor action, signalling and focal adhesion. Thus, a combined platelet proteomics and bioinformatics approach revealed novel insights in regulation of cellular biomarkers indicating that cis9,trans11-CLA may inhibit pathways involved in atherogenesis and thrombosis.
Last updated 2013-09-26

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